Studies on the Effectiveness of Ozone Therapy on the Treatment of Experimentally Induced Keratitis with Candida albicans in Rabbits.

PURPOSE: In the present study, antifungal activity of ozonated olive oil (OZO) and ozonated distilled water (ODW) in the treatment of experimentally induced keratitis with C. albicans in rabbits were investigated. METHODS: The Groups were composed of as 1, 2, 3, 4, 5, 7 (n = 5 rabbits, 10 eyes/in each group) and Group 6 (n = 10 rabbits, 20 eyes/in the group). Fourty-eight hours after C. albicans inoculation; Group 1 received fluconazle (FLU)+OZO drops, Group 2 received FLU drop, Group 3 received OZO drop, Group 4 received FLU+ODW drops, Group 5 received ODW drop, Group 6 (infected control group) and Group 7 received PBS drop (negative control group). Treatment continued in all groups for 22 days for every 8 hours. RESULTS: Cornea cultures made 24 days post inoculation revealed statistically significant differences (p < 0,05) with concern to C. albicans amounts between Group 6 and Group 1-5. Statistical comparison of corneal opacity and corneal ulcer and conjunctivitis values among the Group 6 and Group 1-5 were also different significantly (p < 0,05) on days 20 and 24 post inoculation. CONCLUSION: OZO and ODW were found to be effective in treating C. albicans keratitis in the present study. It has also been proven by this study that ODW contain 26 µg/ml was the most effective in the treatment of C. albicans keratitis.

An echocardiographic study of breed-specific reference ranges in healthy French Bulldogs.

Echocardiography is a standard diagnostic tool for assessment of cardiac functions and cardiovascular diseases in dogs, however published echocardiographic measurements have varied widely based on dog breeds. The objective of this prospective reference interval study was to provide breed-specific echocardiographic values for healthy French Bulldogs. A total of 42 healthy French Bulldogs of both sexes (23 females and 19 males) were sampled. Furthermore, measurements for a control group (n = 16) were also conducted in four other dog breeds (Cocker Spaniel [n = 2], Cavalier King Charles Spaniel [n = 4], Terrier [n = 5], and Crossbreed [n = 5]). Standard M-mode, two-dimensional (2D), pulse wave (PW) Doppler, and tissue Doppler imaging (TDI) echocardiographic measurements were obtained from healthy French Bulldogs. The M-mode echocardiographic data obtained from French Bulldogs were compared to the data obtained from the control group. The left ventricular internal dimension at end-diastole (LVIDd; cm)/body surface area (BSA) (m2 ) ratio for the study group was 3.35/0.53 = 6.32. Left ventricular measurements for French bulldogs and internal dimension at end-systole (21.23 ± 3.50 mm) and at end-diastole (33.50 ± 4.12 mm) were found to be significantly higher (P < .001) compared to control group values (left ventricular internal dimension at end-systole [LVIDs]; 17.46 ± 2.85 mm, LVIDd; 27.16 ± 4.20 mm, respectively). A significantly positive correlation in the French Bulldog group was noted between body weight and M-mode measurements (EPSS, IVSd, IVSs, LVIDd, LVIDs, and LVPWd). French bulldogs had a greater systolic and diastolic left ventricular volume than the control group. As a result, values reported in this study could be used as specific reference ranges in French Bulldogs.

Comparison of Systemic Effects of Midazolam, Ketamine, and Isoflurane Anaesthesia in Rabbits.

INTRODUCTION: Clinical doses of anaesthetic agents were administered to rabbits and effects on the brain, heart, and liver were investigated biochemically and histopathologically. MATERIAL AND METHODS: The rabbits were randomly divided into three main groups (16 rabbits each) and each group into study (n = 8) and control (n = 8) groups. All study group rabbits received 3 mg/kg of midazolam (M) intramuscularly. Group 1.1 (M) received nothing further, group 2.1 (MK) also received 25 mg/kg of ketamine, and group 3.1 (MKI) besides ketamine was also given 2% isoflurane to induce anaesthesia for 30 min. NaCl solution in the same volume as midazolam and ketamine was injected into the controls. RESULTS: In clinical evaluation significant differences were detected in respiratory and heart rates. In blood gas analysis the PO2 and PCO2 values showed statistical differences in anaesthesia intervals. Significant biochemical value changes were recorded in creatine kinase-Mb, glucose, and total protein. Histopathological liver examinations revealed higher total apoptotic and normal cell numbers in the MK than in the M and MKI groups. Apoptotic cell numbers were statistically significant in M and MK groups. CONCLUSION: Anaesthetic agents may increase programmed apoptosis. The MKI anaesthetics combination was found to cause less cell destruction in general than the other study groups. It was indicated that MKI was the safer anaesthetic combination in rabbits.

Neonatal calf meningitis associated with Streptococcus gallolyticus subsp. gallolyticus.

Here, we report a case of neonatal calf meningitis due to Streptococcus gallolyticus subsp. gallolyticus (SGG). Clinical, pathological and microbiological findings were evaluated. API Strep, 16S rRNA gene sequencing, rpoB gene sequencing and sodA gene sequencing were used for the complete identification of SGG. This is the first documented report of neonatal calf meningitis due to SGG in veterinary medicine.

Changes in serum mineral concentrations, biochemical and hematological parameters in horses with pica.

The aim of this study was to compare hematological, some biochemical parameters, and serum trace element concentrations in horses with or without pica. Fifteen horses with pica (group I) and another 15 healthy horses without pica (group II) were used. The hematological parameters were not changed between the two groups. In group I, hemoglobin values were lower than those of group II. However, the difference in hemoglobin values between the two groups was not significant (P > 0.05). Serum iron and copper concentrations and the copper/zinc ratio were lower in group I than those of group II (P < 0.05). The other biochemical parameters were not found to be statistically different between the two groups (P > 0.05). It was concluded that serum iron and copper deficiency may play an important role for the etiology of pica in horses. Prophylactic use of iron and copper supplements in horses may be beneficial to prevent pica.

Detection of myocardial degeneration with point-of-care cardiac troponin assays and histopathology in lambs with white muscle disease.

The aim of this study was to evaluate the use of human cardiac troponin-I (cTn-I) and cardiac troponin-T (cTn-T) kits for the determination of myocardial degeneration in lambs suffering from white muscle disease (WMD). Cardiac troponin (cTn) analyses and necropsy were performed on 12 lambs with acute WMD. Only cTn analyses were tested in six healthy lambs. cTn-I and cTn-T tests were positive for all lambs with WMD, but negative in healthy lambs. Necropsy revealed that the cardiac and skeletal muscles of lambs with WMD had chalky white lesions, which appeared as necrosis and calcification in histopathology. The histopathological findings of the heart muscle and increased cTn in lambs with WMD suggested that marked myocardial degeneration may be detected by point-of-care cTn assays in lambs.

[The investigation of short term efficiency of oxfendazole + oxyclozanide paste and tablet formulations against gastrointestinal nematodes in sheep]. / Koyunlarda Mide-Bagirsak Nematodlarina Karsi Oxfendazole + Oxyclozanide'nin Tablet ve Pasta Formülasyonlarinin Kisa Süreli Etkinliginin Arastirilmasi.

The aim of this study was to investigate the effectiveness of tablet and paste formulations of Oxfendazole and Oxyclozanide combinations against subclinical gastrointestinal nematode infections and to compare the advantages and/or disadvantages of their use. Seventy-five infected sheep were selected from an enterprise located in Kayseri in 2006. The sheep were divided into 3 equal groups as paste, tablet and control groups. Fecal samples were collected from each group before drug administration. While the paste and tablet groups were administered drugs orally, no drugs were given to the controls. Fecal samples were collected on the 7th, 14th, 21st, and 28th days after drug application and the EPG values were determined. The parasitological examination revealed that the most prevalent species was Ostertagia spp., followed by Nematodirus spp. and Trichostrongylus spp. While the mean EPG value of the control group increased up to a ratio of 7.8% at day 28, the mean EPG values of drug groups decreased to 0%. Although the unit dose of paste formulation is more expensive, it was found that it could be an alternative to tablet formulation and has some advantages such as being easier to give, effective utilizing, shorter application period, fewer complications and death risk, no application failure and requires fewer personnel.

Comparison of ventral coccygeal arterial and jugular venous blood samples for pH, pCO2, HCO3, Be(ecf) and ctCO2 values in calves with pulmonary diseases.

The aim of this study was to determine whether venous blood samples can be used as an alternative to arterial samples in calves with respiratory problems and healthy calves. Jugular vein and ventral coccygeal artery were used to compare blood gas values. Sampling of the jugular vein followed soon after sampling of the ventral coccygeal artery in healthy calves (group I) and calves with respiratory problems (group II). Mean values of arterial blood for pH, pCO2, HCO3act in healthy calves were 7.475 +/- 0.004, 4.84 +/- 0.2 kPa, 28.45 +/- 1.30 mmol/L compared with venous samples, 7.442 +/- 0.006, 6 +/- 0.3 kPa, 30.93 +/- 1.36 mmol/L, respectively. In group II, these parameters were 7.414 +/- 0.01, 5.93 +/- 0.3, 27.73 +/- 1.96 mmol/L for arterial blood and 7.398 +/- 0.008, 6.85 +/- 0.2 kPa, 29.77 +/- 1.91 mmol/L for venous blood, respectively. There were no statistically significant differences between arterial and venous pH, HCO3act, Be(ecf), ctCO2 values with the exception of pCO2 (P = 0.001) in group II. In group I, correlation (r2) between arterial and venous blood pH, pCO2, HCO3act were 84.5%, 87.5%, 95.7%, respectively compared with the same parameters in group II, 80.8%, 77.1%, 70.3%. In conclusion, venous blood gas values can predict arterial blood gas values of pH, pCO2 and HCO3ecf, Be(ecf) and ctCO2- for healthy calves but only pH values in calves with acute respiratory problems (r2 value>80%).

Effect of time delay and storage temperature on blood gas and acid-base values of bovine venous blood.

The aim of this study was to investigate possible changes in the gas composition and acid-base values of bovine venous blood samples stored at different temperatures (+4, 22 and 37 degrees C) for up to 48 h. Five healthy cattle were used in the study. A total of 15 blood samples collected from the animals were allocated into three groups, which were, respectively, then stored in a refrigerator adjusted to +4 degrees C (Group I, n=5), at a room temperature of about 22 degrees C (Group II, n=5) and in an incubator adjusted to 37 degrees C (Group III; n=5) for up to 48 h. Blood gas and acid-base values were analysed at 0 (baseline), 1, 2, 3, 4, 5, 6, 12, 24, 36 and 48 h of storage. A significant decrease (p<0.001) was found, in the pH of the refrigerated blood after 5 h and its maximum decrease was recorded at 48 h as 0.04 unit. There were also significant alterations (p<0.001) in the blood pH of the samples stored at room temperature and in the incubator after 2 and 3 h, respectively. The maximum mean alteration in pCO(2) value for Group I was -0.72 kPa during the assessment, while for groups II and III, maximum alterations in pCO(2) were detected as +2.68 and +4.16 kPa, respectively. Mean pO(2) values increased significantly (p<0.001) for Group I after 24 h and for Group II after 6 h, while a significant decrease was recorded for Group III after 24 h (p<0.001). Base excess (BE) and bicarbonate (HCO(3)) fractions decreased significantly for all the groups during the study, compared to their baseline values. In conclusion, acid-base values of the samples stored at 22 and +4 degrees C were found to be within normal range and could be used for clinical purposes for up to 12 and 48 h, respectively, although there were small statistically significant alterations.